Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: Comparison with denaturing gradient gel electrophoresis and nucleotide sequencing
نویسندگان
چکیده
منابع مشابه
Conformation sensitive gel electrophoresis for simple and accurate detection of mutations: comparison with denaturing gradient gel electrophoresis and nucleotide sequencing.
Previously, an assay called conformation sensitive gel electrophoresis (CSGE) was developed for scanning PCR products for the presence of single-base and larger base mismatches in DNA. The assay was based on the assumption that mildly denaturing solvents in an appropriate buffer can accentuate the conformational changes produced by single-base mismatches in double-stranded DNA and thereby incre...
متن کاملThe Comparison of the Effectiveness of a Modified Conformation Sensitive Gel Electrophoresis with Denaturing High Performance Liquid Chromatography
Background: Several methods have been developed for detection of sequence variation in genes and each has its advantages and disadvantages. A disadvantage of them is that the simpler, cost-effective methods are commonly perceived as being less sensitive in their detection of sequence variation, whereas those with proven sensitivity have a requirement for complex or expensive laboratory equipmen...
متن کاملDiversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...
متن کاملDiversity of eukaryotic plankton of aquaculture ponds with Carassius auratus gibelio, using denaturing gradient gel electrophoresis
PCR-denaturing gradient gel electrophoresis (DGGE) and canonical correspondence analysis (CCA) were used to explore the relationship between eukaryotic plankton community succession and environmental factors in two aquaculture pond models with gibel carp Carassius auratus gibelio. The main culture species of pond 1 were gibel carp and grass carp, and the combined density was 46224 fingerling/ha...
متن کاملOnline tool for analysis of denaturing gradient gel electrophoresis profiles.
We present an online tool (EquiBands, http://www.univie.ac.at/IECB/limno/equibands/EquiBands.html) that quantifies the matching of two bands considered to be the same in different samples, even when samples are applied to different denaturing gradient gel electrophoresis gels. With an environmental example we demonstrate the procedure for the classification of two bands of different samples wit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1998
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.95.4.1681